Combination of active peptides and proteins that provide an efficient treatment to restore the collagen levels of youth and mature skin, maintaining an adequate long-lasting collagen function that will ensure a healthy and youthful skin.Appearance
Water (Aqua), Pseudoalteromonas Ferment Extract, Hydrolyzed Wheat Protein, Hydrolyzed Soy Protein, Tripeptide-10 Citrulline, Tripeptide-1, Lecithin, Xanthan Gum, Carbomer, Triethanolamine, Caprylyl Glycol.Dosage 1-5%
During the aging process, the synthesis of collagen is reduced and both degradation and disorganisation of the fibril network are increased, resulting in connective tissue damage, and the loss of the skin three-dimensional integrity, culminating in the development of wrinkles. trylagen®PCB acts at these three stages in the life of collagen and its activity is made up of three main functions: boosts the synthesis of Collagen types I, III and IV, controls collagen fibril dimensions and inhibits enzymatic destruction, avoiding excessive collagen damage in aged skin.Properties
Integral collagen treatment that tackles the problems associated with each aging stage.Applications
trylagen®PCB can be incorporated in cosmetic formulations where attenuation of wrinkles is desired.Solubility Water soluble
A sample of a cream containing 1.25 % Pseudoalteromonas Ferment Extract was tested in reconstituted human skin.
Human Dermal Fibroblasts were treated with a mixture of Hydrolyzed Soy Protein (HSP) and Hydrolyzed Wheat Protein (HWP) at two different concentrations. Collagen III was detected using an ELISA test with monoclonal antibodies.
Tissues from a tridimensional human skin model were treated with Tripeptide-10 Citrulline 0.01%. Tissues were sectioned and then observed by Transmission Electron Microscopy
The aim of this study was to determine the selectivity of trylagen®PCB versus human MMPs: MMP-2 and MMP-3. The fluorescence released by quenched gelatin (denatured collagen) when digested with MMPs was monitored.
Panel of 20 female volunteers, aged 35 to 55.
A cream containing 5% trylagen®PCB was applied twice daily on one side of the face (around the eye), and a placebo cream on the other side, during 30 days. The depth of the wrinkles was examined by means of the optical 3D measurement PRIMOS.
Type I collagen fibrils have a great tensile strength and elastic resistance
Type IV collagen is the most abundant structural component of basement membranes
Youthful skin contains a predominance of collagen III, but during the aging process, cells gradually lose their ability to produce this type of collagen
This function provides a better cohesion and stabilisation of collagen fibres, and gives suppleness to the skin
Its anticollagenase activity protects collagen from degradation and avoids excessive collagen damage in aged skin
The activity of the GluCare® S formulation is expressed as percentage protection or restoration (the maximum damage is the difference in the number of Langerhans Cells in skin which was, and was not subject to UV radiation and not treated with the GluCare® S containing formulation).
As can be seen from the results GluCare® S was extremely effective in protecting the Langerhans Cells against the damaging effects of UV radiation; the reduction in the number of Langerhans Cells could be reduced to approximately 10 %.
Application of the GluCare® S containing formulation resulted even in the partly restoration of the number of Langerhans Cells after this number was sharply reduced as a result of UV radiation.
GluCare® S can be easily incorporated into an O/W emulsion by adding to the water phase before the homogenisation step.
A GluCare® S containing formulation was applied on human skin explants before and after UV radiation (1.5 Joules/cm²). After completing the treatment the number of Langerhans Cells was determined and compared [fig 2 (a and b)].
A perfect facial contour, firm body and reduced wrinkles are all signs of youthfulness that people want to maintain or restore.
With age, the skin loses its elasticity and firmness. Cell turnover slows down, collagen production decreases. But above all, the supporting tissue is disorganized, leading to slackening skin and the appearance of increasingly deep wrinkles.
Today Gattefossé targets cellular dynamism to reinforce the dermis architecture and fight the signs of age
A new, concentrated firming and wrinkle smoothing active ingredient, GATuline® in-Tense acts on the skin natural lifting properties.
By stimulating the fibroblast biomechanical functions, GATuline® in-Tense will reorganize the dermis architecture. The skin density and firmness are then rapidly enhanced, leading to a reduction in skin roughness
GATuline® in-Tense is a concentrated oily extract from the upper part of the Spilanthes acmella plant (Asteraceae family), also called Paracress.
This plant, which mainly grows in subtropical regions, is widespread and appreciated for its flavour enhancement properties.
its French name, "brède mafane", a cross between French and Madagascar Creole, means "hot grass". This name comes directly from its use as a condiment.
Gattefossé harvests the flower buds of Paracress used for GATuline® in-Tense in south Africa.
This origin was specifically selected for the high content of active ingredients in its crops.
its main components are alkylamides, known in traditional medicine for their analgesic and immuno-stimulating effects.
These amides have proved to be excellent reproducibility and activity markers of GATuline® in-Tense. They are guaranteed with a minimum content of 0.5%.
When sourcing new plants, Gattefossé puts a strong focus on protecting the environment.
in the case of GATuline® in-Tense, only the floral part of paracress is picked. The integrity of the plant is therefore maintained, allowing long-term continual development.
Thereby, the specific extraction process allows the recycling of plant residues, which are then used for compost in agriculture.
To boost and develop the local economy, Gattefossé has selected farmers supported by BEE (Black economic empowerment).
This program, launched by the south African government, aims to redress imbalances inherited from Apartheid, by giving minorities economic opportunities to which they did not hitherto have access.
The Extra Cellular Matrix (eCM), the supporting tissue for the skin, plays a major structural role on the skin architecture.
Skin architecture. The eCM can be simply described as a "dermal gel" consisting of water, glycosaminoglycanes (GAGs) and proteoglycanes, in which bathe fibroblasts and protein fibers, mainly collagen and elastin.
Fibroblasts, key cells in the dermis, permanently interact with collagen fibers to create a genuine threedimensional network.
Through their mobility and contractile properties, fibroblasts organize this supporting network by pulling on the collagen fibers.
This cellular dynamism reorganizes and tightens this dermal web, thus firming the skin.
With age, all the components of the eCM are diminished. Cellular dynamism, like all the interactions between cells and supporting fibers, is reduced.
The result is a disorganization of the collagen network. The skin loses its mechanical properties and reveals the signs of aging.
GATuline® in-Tense has demonstrated powerful action to boost this cellular dynamism and thus reorganize and tighten this collagen fiber network.
Tightening and firming activity has been tested on a living artificial dermis model. These lattices are composed of human fibroblasts interlinking in a network of collagen fibers as within the skin.
The effect of GATuline® in-Tense on the dynamic properties of the fibroblasts is evaluated by measuring the contraction surface of the lattices.
Cytokine TGFß, known for its stimulating power on human fibroblasts, has been used as a positive control.
like TGFß, GATuline® in-Tense reduces the surface of equivalent dermis at all measured times (Fig. 1)
The contraction kinetics of the treated dermis is more rapid than in the control batch.
Efficacy is evaluated over 7 days
But the results are visible from the first day with a quicker lattice contraction for the treated one in comparison with untreated lattices (Fig. 2).
These results highlight a clear stimulation of the contractile power of the fibroblasts in the presence of GATuline® in-Tense, in a dose-dependent way, over the range of tested concentrations (Table 1).
This stimulation, obtained after direct addition of the active ingredient in the culture, indicates that GATuline® in-Tense acts on cell/collagen fiber interactions.
These interactions guarantee better cohesion and therefore well structured dermal architecture.
The observed tightening and firming effect was then confirmed during a clinical study on 28 volunteers.
The in vivo wrinkle smoothing activity of GATuline® in-Tense was demonstrated on crow’s feet of women aged 45 to 65.
Tests were conducted on two panels of 28 volunteers each, with twice-daily application for 28 days.
The first panel applied a placebo and the other panel tested a formulation containing 2% GATuline® in-Tense.
The wrinkle smoothing effect of GATuline® in-Tense was visually assessed by Dermatologists during the treatment.
Right from the first day, GATuline® inTense gives perceptible results on the skin microrelief. These results are even clearer after 28 days of treatment.
Before/after photos show the smoothing action of GATuline® in-Tense, capable of visibly reducing the wrinkled appearance of the skin.
This action not only affects the microrelief but also the deepest wrinkles, the most marked ones on the face.
The laser profilometry technique is used to precisely quantify roughness variations at different measuring times.